Testagen

Testagen is the Lys-Glu-Asp-Gly tetrapeptide examined in nucleic-acid interaction experiments and copper-surface chemistry.

Identity

Sequence

H-KEDG-OH

Residues

4

Molecular formula

C₁₇H₂₉N₅O₉

Average molecular weight

447.44 g/mol

Monoisotopic mass (calculated)

447.1965 Da

CAS number

Not recorded

Formula and average mass are computed from the verified free KEDG sequence. CAS, PubChem CID and UniProt were not verified; SMILES and 2D coordinates were not transcribed. Biological literature is limited and mostly from one group; the copper-surface study provides an independent chemistry context.

Identity data from: PubMed PMID 40807317: H-Lys-Glu-Asp-Gly-OH identity · computed from the sequence

Research overview

Testagen is the four-residue sequence Lys-Glu-Asp-Gly, abbreviated KEDG. The terminal glycine differentiates it from KEDP, while the first three residues are KED. A cell-based paper explicitly names Testagen and prints the sequence; the later chemistry article specifies the free H-Lys-Glu-Asp-Gly-OH molecule. These identities permit a sequence-based formula calculation, but the short name does not establish a receptor or an organ-selective mechanism. The two references examine very different settings and must be interpreted within their own designs [1][2].

In the biological study, fluorescence-labelled Testagen was examined in HeLa cells alongside other short peptides. The investigators observed cellular and nuclear fluorescence and measured quenching with labelled oligonucleotides and DNA complexes. The experiment separates localisation of tagged material from interactions measured in cell-free nucleic-acid systems; fluorescence does not alone establish a downstream regulatory pathway [1]. The independent chemistry study investigated KEDG adsorption on copper surfaces in a saline environment using electrochemical measurements, optical microscopy and SEM/EDS. Density-functional calculations and Monte Carlo simulation were also used to examine possible adsorption configurations. These are material-surface observations and computational models, not measurements of a biological organ. They expand the experimental contexts in which KEDG appears without validating biological claims associated with its name [2].

The biological evidence remains limited and mostly linked to one originating group. The surface-chemistry paper is an independent line of work. Nuclear fluorescence, oligonucleotide quenching and copper adsorption should remain separate observations rather than being joined into a common mechanism [1][2].

Laboratory storage and handling

Follow the storage conditions stated in the documentation supplied with the lot. Keep the sealed vial cold, dry and protected from light. Let it reach room temperature before opening to reduce moisture condensation on the material. Avoid repeated freezing and thawing of laboratory stocks, record lot identifiers with experiments and wear standard laboratory protective equipment. Published biological observations do not establish storage stability for a particular lot.

Questions about Testagen

References

1. Penetration of short fluorescence-labeled peptides into the nucleus in HeLa cells and in vitro specific interaction of the peptides with deoxyribooligonucleotides and DNA. Biochemistry (Mosc) 2011. PMID 22117547
2. The Inhibitory Effect and Adsorption Properties of Testagen Peptide on Copper Surfaces in Saline Environments: An Experimental and Computational Study. Molecules 2025. PMID 40807317

Identity data checked on 2026-10-03. Literature summaries describe published research models and are not specifications of any product lot.