PE22-28

PE-22-28 is a seven-residue fragment derived from spadin and studied in TREK-1 potassium-channel experiments.

Identity

Sequence

H-GVSWGLR-OH

Residues

7

Molecular formula

C₃₅H₅₅N₁₁O₉

Average molecular weight

773.9 g/mol

Monoisotopic mass (calculated)

773.4184 Da

CAS number

1801959-12-5

PubChem CID

165437303

Other names

PE 22-28

This identity is the free heptapeptide. An acetate name also occurs in PubChem synonyms, but acetate is absent from the stated formula. No fragment-specific UniProt record or intact SMILES representation was verified.

Identity data from: PubChem CID 165437303

Research overview

PE-22-28 is the heptapeptide GVSWGLR, a shortened sequence derived from spadin. Spadin itself comes from the sortilin propeptide, and the numbered fragment identifies a particular part of that sequence. The primary study developed the shorter peptide while examining products of spadin degradation. This structural relationship places the compound within a defined series of peptide analogues rather than establishing that all members are interchangeable [1].

Investigators used patch-clamp recordings in HEK cells expressing human TREK-1 to examine potassium-channel inhibition. They also compared derivatives with changes at the amino or carboxyl terminus, finding that terminal modifications could alter the recorded channel responses. The same study included cultured mouse cortical neurons and mouse experimental models. These systems supplied different measurements: electrical current, cellular protein markers and behavior in a specified assay cannot be read as the same endpoint [1].

A later review describes shortening, cyclization and retro-inverso design within the broader spadin programme. Its discussion helps distinguish the parent peptide from modified sequences and places TREK-1 experiments in their research context. Findings from a particular derivative belong to that derivative and its experimental conditions; they do not establish a general property of every peptide carrying the spadin name [2].

Laboratory storage and handling

Keep the sealed vial cold, dry and protected from light, following the conditions in the documentation supplied with the lot. Let the vial reach room temperature before opening to limit moisture condensation. Avoid repeated freezing and thawing of laboratory stocks. Record the lot identifier and the material form with each experiment, and use standard laboratory protective equipment.

Questions about PE-22-28

References

1. Shortened Spadin Analogs Display Better TREK-1 Inhibition, In Vivo Stability and Antidepressant Activity. Front Pharmacol 2017. PMID 28955242
2. Fighting against depression with TREK-1 blockers: Past and future. A focus on spadin. Pharmacol Ther 2019. PMID 30291907

Identity data checked on 2026-10-03. Literature summaries describe published research models and are not specifications of any product lot.