PNC 27

PNC-27 is a 32-residue chimeric peptide joining a p53 MDM2-binding segment to a penetratin-derived membrane-residency sequence.

Identity

Sequence

H-PPLSQETFSDLWKLLKKWKMRRNQFWVKVQRG-OH

Residues

32

Molecular formula

C₁₈₈H₂₉₃N₅₃O₄₄S

Average molecular weight

4032 g/mol

Monoisotopic mass (calculated)

4029.2040 Da

CAS number

1159861-00-3

PubChem CID

16201774

Strengths

5–10 mg

The free peptide combines p53 residues 12–26 with a 17-residue penetratin leader. Fluorescently labeled derivatives are separate identities. No fragment-specific UniProt entry or intact SMILES representation was verified.

Identity data from: PubChem CID 16201774 · PubMed PMID 11606716 and linked full text PMC60072

Research overview

PNC-27 combines a segment of the p53 amino-terminal MDM2-binding region with a penetratin leader sequence. The original sequence study distinguishes this 32-residue chain from related constructs containing shorter p53 segments. That design establishes a chimeric peptide rather than an intact p53 protein [1]. A later two-dimensional NMR study examined PNC-27 in aqueous and membrane-mimetic environments. It described different helical arrangements, including an ensemble with a helix–coil–helix organization in the membrane-mimetic system. The reported conformations depend on the experimental environment and should not be treated as a single universal structure [2].

Cell studies separately examined the relation between PNC-27 and membrane-associated HDM-2, the human MDM2 protein. The third cited paper used the K562 leukemia cell line, which lacks p53 expression, and murine leukocytes as a comparison. Investigators measured peptide–protein colocalization by microscopy and used lactate dehydrogenase release and caspase measurements as distinct cellular readouts. This work concerns a particular cultured-cell system and a proposed membrane interaction. It does not establish that all cells display the same protein localization or respond in the same way. Sequence design, NMR conformation and cell-based observations therefore represent complementary but separate levels of evidence [3].

Laboratory storage and handling

Keep the sealed vial cold, dry and protected from light, following the conditions in the documentation supplied with the lot. Let the vial reach room temperature before opening to limit moisture condensation. Avoid repeated freezing and thawing of laboratory stocks. Record the lot identifier and the material form with each experiment, and use standard laboratory protective equipment.

Questions about PNC-27

References

1. Peptides from the amino terminal mdm-2-binding domain of p53, designed from conformational analysis, are selectively cytotoxic to transformed cells. Proc Natl Acad Sci U S A 2001. PMID 11606716
2. NMR solution structure of a peptide from the mdm-2 binding domain of the p53 protein that is selectively cytotoxic to cancer cells. Biochemistry 2004. PMID 14967026
3. The anti-cancer peptide, PNC-27, induces tumor cell necrosis of a poorly differentiated non-solid tissue human leukemia cell line that depends on expression of HDM-2 in the plasma membrane of these cells. Ann Clin Lab Sci 2014. PMID 25117093

Identity data checked on 2026-10-03. Literature summaries describe published research models and are not specifications of any product lot.