Triptorelin

Triptorelin is an amidated GnRH decapeptide analogue with D-tryptophan at residue 6, examined in receptor-binding models.

Identity

Sequence

H-[pGlu]HWSY[D-Trp]LRPG-NH2

Residues

10

Molecular formula

C₆₄H₈₂N₁₈O₁₃

Average molecular weight

1311.4 g/mol

Monoisotopic mass (calculated)

1310.6309 Da

CAS number

57773-63-4

PubChem CID

25074470

Other names

D-Trp6-GnRH

Values describe the free peptide, excluding salts. pGlu means pyroglutamate, and D-Trp identifies the D-tryptophan substitution at residue 6. No separate UniProt accession was verified. A residue diagram is used instead of SMILES and atomic coordinates; blend components do not apply.

Identity data from: PubChem CID 25074470

Research overview

Triptorelin is a synthetic decapeptide analogue of GnRH. It retains an N-terminal pyroglutamate and a C-terminal amide, while D-tryptophan replaces glycine at position 6. This substitution is part of the peptide identity and should remain explicit in a sequence representation. The cited work examines this ligand in GnRH receptor studies, using both gonadotroph-derived cells and receptor-binding assays [1][2].

One investigation used the alphaT3-1 cell line to measure GnRH receptor messenger RNA. It compared continuous and intermittent ligand exposure patterns, and examined estradiol and progesterone as additional experimental variables. Northern blot analysis provided the transcriptional readout. The reported receptor-expression patterns depended on the cellular conditions, so this experiment concerns regulation in a defined cell model rather than a general property inferred from the peptide sequence [1].

A separate study compared the binding kinetics of twelve GnRH peptide agonists. A radiolabelled triptorelin probe supported competition association measurements at the human GnRH receptor, with a time-resolved FRET method providing an alternative assay. The authors compared kinetic measurements with equilibrium affinity and found that those descriptions did not capture identical aspects of binding. For research interpretation, the distinction separates ligand association and dissociation from receptor expression: the two cited studies investigate different levels of the same receptor system [2].

Laboratory storage and handling

Store sealed peptide vials under the temperature conditions stated in the lot documentation, protected from light and moisture. Let a cold vial reach room temperature before opening to limit condensation. Avoid repeated freezing and thawing of laboratory stocks, record storage history and the lot identifier, and use standard laboratory protective equipment. Chemical modifications and counterions should be checked against the supplied analytical description.

Questions about Triptorelin

References

1. Regulation of GnRH I receptor gene expression by the GnRH agonist triptorelin, estradiol, and progesterone in the gonadotroph-derived cell line alphaT3-1. Endocrine 2006. PMID 17185802
2. Characterization of 12 GnRH peptide agonists - a kinetic perspective. Br J Pharmacol 2016. PMID 26398856

Identity data checked on 2026-10-03. Literature summaries describe published research models and are not specifications of any product lot.